Super-multiplexed vibrational imaging for complex biomedicine (Conference Presentation)

The rapidly increasing need for systems biology stimulates the development of super-multiplexed methods for simultaneous imaging multiple biomolecules with distinct colors. The consensus is that complex biology requires multiplex technology. Fluorescent microscopy has inherent limitation due to the fundamental color barrier. In this end, vibrational imaging coupled with novel probes has the potential to address this grand challenge. Here we will present our recent advance along this direction including novel probe development, new labeling and amplification protocols, and application to tissue imaging. Exciting results on super-resolution super-multiplex imaging and high-dimensional single cell analysis will be discussed.