佐剂
炭疽疫苗
体内
化学
炭疽杆菌
CpG寡核苷酸
褐藻糖胶
细胞毒性
体外
微生物学
免疫学
生物
生物化学
多糖
dna疫苗
DNA甲基化
生物技术
细菌
基因表达
基因
遗传学
重组DNA
作者
Meng-Hung Tsai,Chuan-Chang Chuang,Cheng-Cheung Chen,Hui-ju Yen,Kuang‐ming Cheng,Xin-An Chen,Huey-Fen Shyu,Chia-ying Lee,Jenn‐jong Young,Jyh-Hwa Kau
标识
DOI:10.1016/j.carbpol.2020.116041
摘要
Fucoidan/trimethylchitosan nanoparticles (FUC-TMC-NPs) have the potential to improve the immunostimulating efficiency of anthrax vaccine adsorbed (AVA). FUC-TMC-NPs with positive (+) or negative (–) surface charges were prepared via polyelectrolyte complexation, both charged NP types permitted high viability and presented no cytotoxicity on L929, A549 and JAWS II dendritic cells. Flow cytometry measurements indicated lower (+)-FUC-TMC-NPs internalization levels than (–)-FUC-TMC-NPs, yet produced high levels of pro-inflammatory cytokines IFN-γ, IL12p40, and IL-4. Moreover, fluorescence microscope images proved that both charged NP could deliver drugs into the nucleus. In vivo studies on A/J mice showed that (+)-FUC-TMC-NPs carrying AVA triggered an efficient response with a higher IgG anti-PA antibody titer than AVA with CpG oligodeoxynucleotides, and yielded 100 % protection when challenged with the anthracis spores. Furthermore, PA-specific IgG1 and IgG2a analysis confirmed that (+)-FUC-TMC-NPs strongly stimulated humoral immunity. In conclusion, (+)-FUC-TMC-NP is promising anthrax vaccine adjuvant as an alternative to CpG.
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