生物
克隆(编程)
诱导多能干细胞
体细胞核移植
遗传学
表观遗传学
DNA甲基化
基因组印记
胚胎
细胞生物学
基因
胚胎干细胞
胚胎发生
基因表达
胚泡
程序设计语言
计算机科学
作者
Dawei Yu,Jing Wang,Huiying Zou,Tao Feng,Lei Chen,Jia Li,Xiaolan Qi,Zhifang Li,Xiaoyue Duan,Chunlong Xu,Liang Zhang,Xi Long,Jing Lan,Chao Chen,Chao Wang,Xinyu Xu,Jilong Ren,Yiqiang Zhao,Xiaoxiang Hu,Zhengxing Lian
标识
DOI:10.1073/pnas.1814514115
摘要
Significance To investigate the epigenetic mechanism of pregnancy failure in mammals, we exploited the high rate of fetal loss in pig induced pluripotent stem cell (iPSC) nuclear transfer. We generated methylomes of pig iPSCs and associated nuclear transfer embryos from reciprocal crosses between two distinct pig breeds. Our methylome analysis revealed that misregulation of RTL1 as the principal basis of pregnancy failure using pig iPSCs. Remarkably, RTL1 has broad fertility implications across mouse, rat, pig, cattle, and human from nuclear transfer cloning, tetraploid complementation, and artificial insemination, to natural fertilization. In all of these procedures, low RTL1 expression consistently corresponds to pregnancy failures.
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