柠檬酸
检出限
同型半胱氨酸
碳纳米管
生物传感器
化学
电极
核化学
无机化学
材料科学
色谱法
纳米技术
生物化学
物理化学
作者
Sara Knežević,Miloš Ognjanović,Marija Gavrović Jankulović,Tatjana Đurašinović,Bratislav Antić,Sladjana Djurić,Dalibor M. Stanković
标识
DOI:10.1002/elan.202100362
摘要
Abstract S‐Adenosyl‐L‐Homocysteine (AdoHcy) is a crucial pathological factor in homocysteine‐associated disorders. We developed an impedimetric approach for single drop S‐Adenosyl‐L‐Homocysteine detection, based on S‐Adenosyl‐L‐Homocysteine hydrolase (SAH), produced in the prokaryotic expression system (E. coli) by recombinant DNA technology, immobilized on citrate acid‐coated gallium oxyhydroxide dispersed over single‐walled carbon nanotubes (CA/GaO(OH)@SWCNT). The proposed biosensor offers detection of AdoHcy, with a limit of detection (LOD) of 0.17 μM. with operating linear range from 1 to 44 μM. The remarkable stability of gallium oxyhydroxide crystal was further improved by citric acid coating and its optical, electrical and physical anisotropy was skipped using SWCNTs.
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