自体荧光
拉曼散射
光子计数
拉曼光谱
光学
相干反斯托克斯拉曼光谱
材料科学
纳秒
分光计
分析化学(期刊)
信号(编程语言)
荧光
光电子学
光子
干涉测量
化学
激光器
物理
色谱法
计算机科学
程序设计语言
作者
Nassim Ksantini,Israël Veilleux,Marie-Maude de Denus-Baillargeon,Patrick Orsini,Isabelle Dicaire,Jean-Bernard Lecourt,Alexandre Gognau,Yves Hernandez,Antonio Baylon,Maroun Massabki,Frédéric Lesage,Frédéric Leblond
标识
DOI:10.1002/jbio.202100188
摘要
Attainable levels of signal-to-background ratio (SBR) in Raman spectroscopy of biological samples is limited by the presence of endogenous fluorophores. It is customary to remove the ubiquitous fluorescence background using postacquisition data processing. However, new approaches are needed to reduce background contributions and maximize the fraction of the sensor dynamical range occupied by Raman photons. Time-resolved detection using pulsed lasers and time-gated measurements can be used to address the signal-to-background problem in biological samples by limiting light detection to nonresonant interaction phenomena with relaxation time scales occurring on sub-nanosecond time scales, thereby excluding contributions from resonant phenomena such as fluorescence. A time-gated Fourier-transform spectrometer was assembled using a commercially available interferometer, a single channel single-photon avalanche diode and time tagging electronics. A time gate of 300 ps increased the signal-to-background-ratio of the 1440 cm-1 Raman band from 36% to 69% in an olive oil sample hereby demonstrating the potential of this approach for autofluorescence suppression.
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