氧化脂质
化学
脂多糖
肝损伤
促炎细胞因子
药理学
多不饱和脂肪酸
生物化学
半乳糖胺
脂肪酸
炎症
酶
免疫学
生物
氨基葡萄糖
作者
Zhikun Zhan,Tao Zhang,Fahong Dai,Wen Xiao,Yulian Chen,Huanguo Jiang,Tanwei Gu,Yuan Cheng,Lan Tang
标识
DOI:10.1016/j.intimp.2021.108387
摘要
Oridonin (Ori) has been shown to protect against acute liver injury (ALI) induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS). Oxylipins are oxidation products of polyunsaturated fatty acids (PUFAs) and are key proinflammatory mediators. This study aimed to investigate the changes in oxylipins in the livers of mice with D-GalN/LPS-induced ALI and the effects of Ori on these changes.54 oxylipins in liver tissues were identified and qualitatively and quantitatively analyzed by ultra-performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (UPLC-QTRAP/MS/MS). The levels of 12-HETE, 12-HEPE, 14(S)-HDHA, PGE2, dihomo-γ-linolenic acid and 13-HOTrE in the liver were significantly increased in the D-GalN/LPS-induced ALI group compared with the control group, and the levels of EPA and 7-HDHA were significantly decreased. However, pretreatment with Ori dramatically decreased the levels of 12-HETE, 12-HEPE, 14(S)-HDHA, PGE2 and 13-HOTrE compared with those of the ALI group and induced 7-HDHA and 15-oxoETE. Moreover, Ori reduced the protein levels of COX-1, COX-2, ALOX5, ALOX12 and ALOX15 induced by D-GalN/LPS, indicating that Ori altered oxylipins through the COX and LOX pathways.These results suggest that the protective effect of Ori on ALI is partly mediated by affecting the oxylipin pathway.
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