蛋白酵素
蛋白酶
计算生物学
蛋白质组学
工作流程
底物特异性
劈理(地质)
计算机科学
化学
基质(水族馆)
纳米技术
生物
生物化学
酶
材料科学
数据库
基因
古生物学
断裂(地质)
生态学
作者
Simonas Savickas,Ulrich auf dem Keller
标识
DOI:10.1515/hsz-2017-0187
摘要
Abstract Targeted degradomics integrates positional information into mass spectrometry (MS)-based targeted proteomics workflows and thereby enables analysis of proteolytic cleavage events with unprecedented specificity and sensitivity. Rapid progress in the establishment of protease-substrate relations provides extensive degradomics target lists that now can be tested with help of selected and parallel reaction monitoring (S/PRM) in complex biological systems, where proteases act in physiological environments. In this minireview, we describe the general principles of targeted degradomics, outline the generic experimental workflow of the methodology and highlight recent and future applications in protease research.
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