衣壳
热稳定性
化学
类病毒颗粒
力谱学
共价键
化学稳定性
材料科学
纳米技术
生物物理学
生物
生物化学
原子力显微镜
重组DNA
有机化学
基因
作者
Zhuohong Wu,Jorge Leganés Bayón,Tatiana B. Kouznetsova,Tetsu Ouchi,Krister J. Barkovich,Sean Kai Hsu,Stephen L. Craig,Nicole F. Steinmetz
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-01-31
卷期号:24 (10): 2989-2997
标识
DOI:10.1021/acs.nanolett.3c03806
摘要
Many virus-like particles (VLPs) have good chemical, thermal, and mechanical stabilities compared to those of other biologics. However, their stability needs to be improved for the commercialization and use in translation of VLP-based materials. We developed an endoskeleton-armored strategy for enhancing VLP stability. Specifically, the VLPs of physalis mottle virus (PhMV) and Qβ were used to demonstrate this concept. We built an internal polymer "backbone" using a maleimide-PEG15-maleimide cross-linker to covalently interlink viral coat proteins inside the capsid cavity, while the native VLPs are held together by only noncovalent bonding between subunits. Endoskeleton-armored VLPs exhibited significantly improved thermal stability (95 °C for 15 min), increased resistance to denaturants (i.e., surfactants, pHs, chemical denaturants, and organic solvents), and enhanced mechanical performance. Single-molecule force spectroscopy demonstrated a 6-fold increase in rupture distance and a 1.9-fold increase in rupture force of endoskeleton-armored PhMV. Overall, this endoskeleton-armored strategy provides more opportunities for the development and applications of materials.
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