柠檬酸杆菌
结肠炎
微生物学
生物
肠道菌群
免疫学
作者
Wanyi Zeng,Jinjie Wu,Hui Xie,Haoming Xu,Danfu Liang,Qilang He,Xinquan Yang,Chen Liu,Junli Gong,Qiang Zhang,Zhanhao Luo,Yuan Chen,Zhen He,Ping Lan
出处
期刊:EBioMedicine
[Elsevier]
日期:2024-02-01
卷期号:100: 104959-104959
标识
DOI:10.1016/j.ebiom.2023.104959
摘要
BackgroundExclusive enteral nutrition (EEN) is an important alternative strategy for patients with Crohn’s disease (CD), and during this process, microbiota alterations have been observed. However, the underlying mechanisms by which EEN reduces intestinal inflammation are currently unclear.MethodsThe therapeutic potential of enteral nutrition (EN) was assessed using various mouse models. Fecal full-length 16S rDNA sequencing analysis and several CD metagenome datasets were used to identify the candidate therapeutic bacteria Faecalibaculum rodentium (F. rodentium). Whole genome sequencing of F. rodentium and widely-targeted metabolome analysis of the supernatant showed that EN-induced F. rodentium accumulation protected against colitis via histidine biosynthesis.FindingsThe therapeutic potential of EN therapy was observed in both dextran sulfate sodium (DSS)-induced colitis and Il10−/− spontaneous colitis mouse models. Accumulation of F. rodentium after EN therapy was determined using full-length 16S rDNA sequencing and verified with several metagenome datasets from patients with CD. Colonization of an isolated F. rodentium could reduce colitis in Il10−/− mice. Significant histidine enrichment was observed in the F. rodentium culture supernatant, and a series of histidine biosynthesis genes were observed in the F. rodentium genome. Engineered Escherichia coli Nissle 1917 (EcN), encoding the heterologous hisG of F. rodentium (EcN-hisG), which was a key driver of histidine biosynthesis in F. rodentium, was found to protect against colitis.InterpretationThis study suggests that EN-induced F. rodentium accumulation protects against colitis in mice via gut bacteria-mediated histidine biosynthesis.FundingA full list of funding bodies can be found in the Acknowledgements section.
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