MTT法
细胞凋亡
癌细胞
流式细胞术
活力测定
细胞周期
糖苷
细胞培养
细胞周期检查点
夹竹桃科
化学
癌症
生物
分子生物学
生物化学
植物
遗传学
作者
Thi Thanh Huong Le,Lei Ma,Phu Hiep Hoang,Van Hung Hoang,Pham Van Khang,Phu Hung Nguyen
出处
期刊:Steroids
[Elsevier]
日期:2023-11-01
卷期号:199: 109310-109310
被引量:1
标识
DOI:10.1016/j.steroids.2023.109310
摘要
C21 steroidal glycosides are a group of natural compounds with biological activities such as anti-cancer, anti-microbial, and anti-viral properties. In this study, we isolated and determined the structure of a new C21 steroidal glycoside, named Marsdenialongise A from Marsdenia longipes W.T. Wang, using nuclear magnetic resonance spectroscopy and mass spectra data. Marsdenialongise A is a derivative of tenacigenin B and was isolated for the first time from a plant. The inhibitory effect of Marsdenialongise A on cancer cells was evaluated through MTT and cell migration assays, cell cycle, and apoptosis analyses. The results of the MTT assay showed that Marsdenialongise A reduces the cell viability of cancer cells, with the AGS cell line being more sensitive than other cell lines, with an IC50 value of 5.69 µM (for 48 h of treatment). Marsdenialongise A also exhibited an ability to prevent the migration of cancer cells in AGS cells. Further analysis using flow cytometry has revealed that Marsdenialongise A is capable of inducing cell cycle arrest and apoptosis. The overexpression of reactive oxygen species (ROS) production induced by Marsdenialongise A can be considered a cause that leads to the influence on the cell cycle and apoptosis of cancer cells. Thus, Marsdenialongise A can be considered a potential anti-cancer agent.
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