生物
夜蛾
保幼激素
蜕皮激素
有害生物分析
基因
幼虫
转录组
昆虫
粘虫
RNA干扰
毒性
基因表达
核糖核酸
遗传学
植物
内科学
重组DNA
医学
作者
Benshui Shu,Yanzheng Lin,Yu‐Ting Huang,Luyang Liu,Xueming Cai,Jintian Lin,Jingjing Zhang
出处
期刊:Gene
[Elsevier]
日期:2024-01-01
卷期号:893: 147928-147928
被引量:1
标识
DOI:10.1016/j.gene.2023.147928
摘要
The fall armyworm, Spodoptera frugiperda, is a destructive agricultural pest that seriously threatens global food security. Insecticide resistance of this pest has gradually formed in recent years due to improper usage, and alternative methods are badly needed. Toosendanin (TSN) is a botanical compound with broad-spectrum insecticidal activities against many pests. However, the effects of TSN on S. frugiperda are still unclear. In this study, the growth inhibition phenomenon, including weight loss and prolonged developmental duration, in the larvae with TSN exposure was clearly observed. Compared to the control group, a total of 450 and 3314 differentially expressed genes (DEGs) were identified by RNA-Seq in the larvae groups treated with 10 and 20 mg/kg TSN, respectively. Furthermore, the DEGs involved in the juvenile hormone and ecdysone signal pathways and downstream processes, including detoxifying enzyme genes, chitin synthesis and metabolism genes, and cuticular protein genes, were found. Our findings suggest that TSN regulates the expression of key genes in juvenile hormone and ecdysone signal pathways and a series of downstream processes to alter the hormone balance and cuticle formation and eventually inhibit larval growth, which laid the foundation for the molecular toxicological mechanism research of TSN on S. frugiperda larvae.
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