Study of Microbial Cytokinin Production Under Various Enriched Conditions and its Effect on Plant Growth

ACKGROUD & OBJECTIVE: The present study highlights the effect of various nitrogen supplements responsiblefor the growth and production of microbial cytokinin. METHODOLOGY: During the experimental process it iscompared with the production of cytokinin by different organisms, viz; Rhizobium sp, Bacillus subtilis BC1,Pseudomonas aeruginosa PAO1 and E.coli K12. The microbial cytokinin thus produced was tested for its effect on plantgrowth and shoot induction in a test plant, Phylodendron xanadu. RESULTS: According to the results observed E.coliK12 synthesized 0.186 mg/L amount of cytokinin in PM2. On the other hand, Bacillus subtilis BC1 showed the leastproduction of cytokinin as compared to the other two. For this organism the amounts produced were 0.044 mg/L in PM1and 0.0075 mg/L in PM2. CONCLUSION: The conclusion may be drawn as that PM1 is good for Rhizobium sp andBacillus subtilis BC1. More shoot numbers was obtained in case of cytokinin sample from E.coli K12 with a heightmeasuring to 3.5 cm in media with activated charcoal and 2.3 cm in media without activated charcoal. Similarly, PM3,PM4, PM5, and PM6 were also tried with various enrichment factors incorporated into the medium and the productionwas compared for cytokinin production.