蛋白质组
生物
生物化学
烟酰胺腺嘌呤二核苷酸磷酸
磷酸戊糖途径
蛋白激酶A
钙调蛋白
糖酵解
激酶
新陈代谢
酶
氧化酶试验
作者
Dharmendra Singh,Shubhra Maithreyi,Jyoti Taunk,Madan Pal Singh
摘要
Abstract Aluminium (Al) toxicity causes major plant distress, affecting root growth, nutrient uptake and, ultimately, agricultural productivity. Lentil, which is a cheap source of vegetarian protein, is recognized to be sensitive to Al toxicity. Therefore, it is important to dissect the physiological and molecular mechanisms of Al tolerance in lentil. To understand the physiological system and proteome composition underlying Al tolerance, two genotypes [L‐4602 (Al‐tolerant) and BM‐4 (Al‐sensitive)] were studied at the seedling stage. L‐4602 maintained a significantly higher root tolerance index and malate secretion with reduced Al accumulation than BM‐4. Also, label‐free proteomic analysis using ultra‐performance liquid chromatography‐tandem mass spectrometer exhibited significant regulation of Al‐responsive proteins associated with antioxidants, signal transduction, calcium homeostasis, and regulation of glycolysis in L‐4602 as compared to BM‐4. Functional annotation suggested that transporter proteins (transmembrane protein, adenosine triphosphate‐binding cassette transport‐related protein and multi drug resistance protein), antioxidants associated proteins (nicotinamide adenine dinucleotide dependent oxidoreductase, oxidoreductase molybdopterin binding protein & peroxidases), kinases (calmodulin‐domain kinase & protein kinase), and carbohydrate metabolism associated proteins (dihydrolipoamide acetyltransferase) were found to be abundant in tolerant genotype providing protection against Al toxicity. Overall, the root proteome uncovered in this study at seedling stage, along with the physiological parameters measured, allow a greater understanding of Al tolerance mechanism in lentil, thereby assisting in future crop improvement programmes.
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