再生(生物学)
细胞生物学
巨噬细胞
瞬态(计算机编程)
利基
成纤维细胞
生物
化学
细胞培养
生态学
生物化学
遗传学
计算机科学
体外
操作系统
作者
Jisheng Sun,Elizabeth A. Peterson,Xin Chen,Jinhu Wang
出处
期刊:Cell Reports
[Elsevier]
日期:2024-04-01
卷期号:43 (4): 114092-114092
标识
DOI:10.1016/j.celrep.2024.114092
摘要
Macrophages conduct critical roles in heart repair, but the niche required to nurture and anchor them is poorly studied. Here, we investigated the macrophage niche in the regenerating heart. We analyzed cell-cell interactions through published single-cell RNA sequencing datasets and identified a strong interaction between fibroblast/epicardial (Fb/Epi) cells and macrophages. We further visualized the association of macrophages with Fb/Epi cells and the blockage of macrophage response without Fb/Epi cells in the regenerating zebrafish heart. Moreover, we found that ptx3a+ epicardial cells associate with reparative macrophages, and their depletion resulted in fewer reparative macrophages. Further, we identified csf1a expression in ptx3a+ cells and determined that pharmacological inhibition of the csf1a pathway or csf1a knockout blocked the reparative macrophage response. Moreover, we found that genetic overexpression of csf1a enhanced the reparative macrophage response with or without heart injury. Altogether, our studies illuminate a cardiac Fb/Epi niche, which mediates a beneficial macrophage response after heart injury.
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