CD19
转录因子
外周血
FOXP3型
生物
免疫学
功能(生物学)
细胞生物学
遗传学
基因
免疫系统
作者
Abraham Cardenas-Juarez,Edith Elena Uresti‐Rivera,Fátima de Lourdes Ochoa-González,Flor Itzel Lira-Hernández,Edgar E. Lara‐Ramírez,Juan Manuel Vargas‐Morales,Bruno Rivas‐Santiago,Diana Patricia Portales‐Pérez,Mariana Haydee García-Hernández
标识
DOI:10.1080/08820139.2025.2515411
摘要
The aim of this study was to evaluate FOXP3 expression in CD19+CD39+ and CD19+CD39- B cells, and to investigate its potential regulatory role. Peripheral B cells were obtained from 25 volunteers. FOXP3 expression at the mRNA and protein levels was analyzed in CD19+CD39+ and CD19+CD39- B cells by FACS and RT-qPCR. Suppressive activity was assessed through co-cultures of PBMC with CD19+CD39+ and CD19+CD39- B cells stimulated with anti-CD3/CD28, evaluating T cell proliferation and the percentage of Th1 cells. The percentage of CD19+CD39+ FOXP3+ B cells was higher compared to other phenotypes. There was a positive correlation between FOXP3 and CD39 in CD19+ B cells. FOXP3 mRNA was increased in CD19+CD39+ B cells compared to CD19+CD39- B cells. CD19+CD39- B cells reduced the proliferation, the percentage of Th1 cells, and expressed higher IL-10 mRNA compared to CD19+CD39+ B cells. B cell phenotypes were inversely associated with Th1 cells and CRP. CD19+CD39- was associated with HOMA-β. CD19+CD39+ was inversely associated with HbA1c. FOXP3 is expressed on both CD19+CD39- and CD19+CD39+ B lymphocytes. CD19+CD39- cells showed high levels of IL-10 and low levels of FOXP3 mRNA. CD19+CD39- B cells decreased the Th1 cells and were associated with β-cell function.
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