纳米颗粒
粒径
化学
血液蛋白质类
粒子(生态学)
蛋白质组学
日冕(行星地质学)
生物物理学
纳米材料
色谱法
纳米技术
材料科学
生物化学
生物
维纳斯
物理化学
基因
天体生物学
生态学
作者
Stefan Tenzer,Dominic Docter,Susanne Rosfa,Alexandra Wlodarski,Jörg Kuharev,Alexander Rekik,Shirley K. Knauer,Christoph Bantz,Thomas Nawroth,Carolin Bier,Jarinratn Sirirattanapan,Wolf J. Mann,Lennart Treuel,R. Zellner,Michael Maskos,Hansjörg Schild,Roland H. Stauber
出处
期刊:ACS Nano
[American Chemical Society]
日期:2011-08-25
卷期号:5 (9): 7155-7167
被引量:736
摘要
In biological fluids, proteins associate with nanoparticles, leading to a protein "corona" defining the biological identity of the particle. However, a comprehensive knowledge of particle-guided protein fingerprints and their dependence on nanomaterial properties is incomplete. We studied the long-lived ("hard") blood plasma derived corona on monodispersed amorphous silica nanoparticles differing in size (20, 30, and 100 nm). Employing label-free liquid chromatography mass spectrometry, one- and two-dimensional gel electrophoresis, and immunoblotting the composition of the protein corona was analyzed not only qualitatively but also quantitatively. Detected proteins were bioinformatically classified according to their physicochemical and biological properties. Binding of the 125 identified proteins did not simply reflect their relative abundance in the plasma but revealed an enrichment of specific lipoproteins as well as proteins involved in coagulation and the complement pathway. In contrast, immunoglobulins and acute phase response proteins displayed a lower affinity for the particles. Protein decoration of the negatively charged particles did not correlate with protein size or charge, demonstrating that electrostatic effects alone are not the major driving force regulating the nanoparticle-protein interaction. Remarkably, even differences in particle size of only 10 nm significantly determined the nanoparticle corona, although no clear correlation with particle surface volume, protein size, or charge was evident. Particle size quantitatively influenced the particle's decoration with 37% of all identified proteins, including (patho)biologically relevant candidates. We demonstrate the complexity of the plasma corona and its still unresolved physicochemical regulation, which need to be considered in nanobioscience in the future.
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