生物
胚胎干细胞
臼齿
翻译(生物学)
搪瓷漆
细胞生物学
胚胎发生
分子生物学
胚胎
遗传学
基因
信使核糖核酸
材料科学
复合材料
古生物学
作者
Thomas G. H. Diekwisch,Shulman David,Pablo Bringas,Salvatore Valentino,Harold C. Slavkin
出处
期刊:Development
[The Company of Biologists]
日期:1993-02-01
卷期号:117 (2): 471-482
被引量:139
标识
DOI:10.1242/dev.117.2.471
摘要
ABSTRACT During tooth development, enamel organ epithelial cells express a tissue-specific gene product (amelogenin) which presumably functions to control calcium hydroxyapatite crystal growth patterns during enamel biomineralization. The present studies were designed to test the hypothesis that amelogenin as a supramolecular aggregate regulates crystal growth during enamel biomineralization. Antisense oligodeoxynucleotide strategy was used in a simple organ culture system to inhibit amelogenin translation. Under these experimental conditions, antisense treatment prior to and during amelogenin expression resulted in inhibition of amelogenin translation products within immunoprecipitated [35S]methionine metabolically labeled proteins. To determine the efficiency of antisense treatment in this model system, digoxigenin-labeled oligodeoxynucleotides were observed to diffuse throughout the tooth explants including the target ameloblast cells within 24 hours. Ultrastructural analyses of amelogenin supramolecular assembly as electron-dense stippled materials in antisense treated cultures demonstrated dysmorphology of the extracelular enamel matrix with a significant reduction in crystal length and width. We conclude that secreted extracellular proteins form a supramolecular aggregate, which controls both the orientation and dimensions of enamel crystal formation during tooth development.
科研通智能强力驱动
Strongly Powered by AbleSci AI