化学
丁酰胆碱酯酶
乙酰胆碱酯酶
变构调节
阿切
抑制性突触后电位
酶
活动站点
对接(动物)
立体化学
生物化学
马尾藻
结构-活动关系
体外
生物
神经科学
藻类
护理部
医学
生态学
作者
Su Hui Seong,Yousof Ali,Hyeung‐Rak Kim,Hyun Ah Jung,Jae Sue Choi
标识
DOI:10.1016/j.bmc.2017.05.033
摘要
A wide range of pharmacological properties of Sargassum spp. extracts and isolated components have been recognized. Although individual meroterpenoids of Sargassum species have been reported to possess strong activity against Alzheimer’s disease (AD), the active compounds of Sargassum serratifolium have not been fully explored. Therefore, we evaluated the anti-AD activity of S. serratifolium extract through enzyme inhibition of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1). Three meroterpenoids (sargahydroquinoic acid (1), sargachromenol (2) and sargaquinoic acid (3)) were isolated from S. serratifolium. These compounds showed moderate AChE inhibitory activity, but exhibited potent inhibitory activity against BChE and BACE1 (15.1, 9.4, and 10.4 µM for BChE; 4.3, 6.9, and 12.5 µM for BACE1, respectively). Kinetic study and molecular docking simulation of these compounds demonstrated that 1 and 3 interacted with both catalytic aspartyl residues and allosteric sites of BACE1, whereas 2 interacted with the allosteric site of BACE1. The results of the present study demonstrate that meroterpenoids from S. serratifolium might be beneficial in the treatment of AD.
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