Experimental Study on Preparation and Properties of Immobilized Tyrosinase on Chitosan Modified by Formaldehyde

Chkosan (activated by glutaraldehyde) , modified by formaldehyde, was prepared and applied to immobilize tyrosinase. The preparation and properties of immobilized tyrosinase on Chitosan was studied. The effects of concentration of glutaraldehyde and formaldehyde, pH and amount of tyrosinase on the immobilized Tyrosinase were investigated. The results show that the best technical condition of formaldehyde modified Chitosan for immobilizing tyrosinase are; the concertration of glutaraldehyde and formaldehyde is 3% and 5% respectively; PH is 8; Amount of tyrosinase is 105 U/g. The immobilized tyrosinase has good biochemical properties.