Study on the Anti-HSV Activity of Crude Extract from Phyllanthus emblica in vitro

Objective To study the active components of anti-HSV-1 and HSV-2 of crude extract from Phyllanthus emblica.Methods The toxic and antivirous effects were evaluated by observing toxic effect,cytopathic effect(CPE),MTT colorimetric assay for vital cell rate,plaques reduction ratio.Results Inhibition on herpes simplex virus were observed during the experiment.The IC50 on HSV-1 of acetic ether extract,methanol extract,aqueous extract from Phyllanthus emblica were 25.28,66.17,100.94μg/ml;while the IC50 on HSV-2 of the extracts above were 31.70,180.3,112.1μg/ml.Conclusion The three extracts from Phyllanthus emblica can inhibit both of HSV-1 and HSV-2 in vitro.