A highly sensitive gold nanosol SERS aptamer assay for glyphosate with a new COF nanocatalytic reaction of glycol-Au(III)

A highly catalytic and stable covalent organic frameworks (COFs) of MaBd were prepared by the polycondensation of melamine (Ma) and p-benzaldehyde (Bd), and characterized by molecular spectra and electron microscopy. MaBd strongly catalyze the new indicator reaction between glycol (GC) and chloroauric acid to generate gold nanoparticles (AuNP) with strong RRS effect at 370 nm and surface enhanced Raman scattering (SERS) effect at 1617 cm−1 using Vitoria blue B (VBB) as molecular probes. Combining the MaBd nanocatalytic amplification reaction with highly selective aptamer (Apt) of glyphosate (GLY), a new, simple and highly sensitive SERS method was developed with a linear range of 0.003−0.07 nmol/L, and the detection limit of 0.002 nmol/L GLY. GLY in soil samples were analyzed by this SERS method, with relative standard deviation of 4.78–5.71 % and recovery of 92.3–105.3 %.