生物
16S核糖体RNA
系统发育树
克莱德
核糖体RNA
拉伤
系统发育学
植物
基因
微生物学
遗传学
解剖
作者
Shuhei Yabe,Yu Zheng,Chiung Mei Wang,Yasuteru Sakai,Keietsu Abe,Akira Yokota,Stefano Donadio,Linda Cavaletti,Paolo Monciardini
标识
DOI:10.1099/ijsem.0.004883
摘要
The aerobic, Gram-positive, mesophilic Ktedonobacteria strains, Uno17 T , SOSP1-1 T , 1-9 T , 1-30 T and 150040 T , formed mycelia of irregularly branched filaments, produced spores or sporangia, and numerous secondary metabolite biosynthetic gene clusters. The five strains grew at 15–40 °C (optimally at 30 °C) and pH 4.0–8.0 (optimally at pH 6.0–7.0), and had 7.21–12.67 Mb genomes with 49.7–53.7 mol% G+C content. They shared MK9(H 2 ) as the major menaquinone and C 16 : 1 -2OH and iso-C 17 : 0 as the major cellular fatty acids. Phylogenetic and phylogenomic analyses showed that Uno17 T and SOSP1-9 T were most closely related to members of the genus Dictyobacter , with 94.43–96.21 % 16S rRNA gene similarities and 72.16–81.56% genomic average nucleotide identity. The strain most closely related to SOSP1-1 T and SOSP1-30 T was Ktedonobacter racemifer SOSP1-21 T , with 91.33 and 98.84 % 16S rRNA similarities, and 75.13 and 92.35% average nucleotide identities, respectively. Strain 150040 T formed a distinct clade within the order Ktedonobacterales , showing <90.47 % 16S rRNA gene similarity to known species in this order. Based on these results, we propose: strain 150040 T as Reticulibacter mediterranei gen. nov., sp. nov. (type strain 150 040 T =CGMCC 1.17052 T =BCRC 81202 T ) within the family Reticulibacteraceae fam. nov. in the order Ktedonobacterales ; strain SOSP1-1 T as Ktedonospora formicarum gen. nov., sp. nov. (type strain SOSP1-1 T =CGMCC 1.17205 T =BCRC 81203 T ) and strain SOSP1-30 T as Ktedonobacter robiniae sp. nov. (type strain SOSP1-30 T =CGMCC 1.17733 T =BCRC 81205 T ) within the family Ktedonobacteraceae ; strain Uno17 T as Dictyobacter arantiisoli sp. nov. (type strain Uno17 T =NBRC 113155 T =BCRC 81116 T ); and strain SOSP1-9 T as Dictyobacter formicarum sp. nov. (type strain SOSP1-9 T =CGMCC 1.17206 T =BCRC 81204 T ) within the family Dictyobacteraceae .
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