清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

High‐throughput sequencing reveals differential expression of miRNAs in yak and cattleyak epididymis

附睾 生物 小RNA 精子 基因 基因表达 精子发生 男科 基因表达调控 小桶 遗传学 细胞生物学 转录组 内分泌学 医学
作者
Cailian Wang,Tajmal Hussain Solangi,Hongmei Wang,Liuyueling Yang,Khuram Shahzad,Wangsheng Zhao,Xia Lang
出处
期刊:Reproduction in Domestic Animals [Wiley]
卷期号:57 (2): 125-140 被引量:8
标识
DOI:10.1111/rda.13973
摘要

Cattleyaks (CY) are interspecific hybrids between cattle (Bos taurus) and yak (Bos gruniens, YK) exhibiting the same prominent adaptability and higher performances than YK. The main problem of this crossbreeding is that the males are sterile. Different series of events of spermatogenesis coordinate to regulate gene expressing, involving microRNAs (miRNAs). As non-coding ribonucleic acids (ncRNAs), miRNAs predominantly facilitate the regulation of gene expression at post-transcriptional stages and play important roles in the acquisition and maintenance of male fertility in reproduction. The function of miRNA in the male reproductive system extends from the testis into the epididymis, regulating gene expression and contributing to regional gene expression variations. RNA sequencing on biological replicates, we described differentially expressed miRNAs profiles for tissue from epididymis of YK and CY. In the present study, High-throughput sequencing analysis showed that 55 differentially expressed (DE) miRNAs were identified in the epididymis of YK and CY. Among these, 43 DE miRNAs were upregulated while the remaining 12 DE miRNAs were downregulated between epididymis of YK and CY. In addition, we identified that the top most important DE miRNAs, bta-miR-449c, bta-miR-539, bta- miR-136, bta-miR-504, bta-miR-31 and bta-miR-222 were involved in the process of sperm maturation in epididymis CY. It was identified that the bta-miR-449c and bta-miR-222 may play major roles in the process of sperm maturation, sperm quality, sperm count, sperm production and male infertility of CY. Furthermore, GO and KEGG analyses were performed to classify the functions of target genes for DE miRNAs. In addition, RT-qPCR validation of the DE miRNAs and its targeted genes revealed that putative miRNAs are involved in the male CY infertility by altering the gene expression. Present findings may not only increase our understanding of the molecular mechanisms regulated by the miRNAs in epididymis, but also provide a valuable information to understand the male infertility mechanism of CY.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
gmc完成签到 ,获得积分10
2秒前
谷子完成签到 ,获得积分10
3秒前
数乱了梨花完成签到 ,获得积分10
11秒前
寒战完成签到 ,获得积分10
19秒前
沐浠完成签到 ,获得积分10
31秒前
salty完成签到 ,获得积分10
40秒前
桐桐应助123采纳,获得10
50秒前
Shandongdaxiu完成签到 ,获得积分10
53秒前
HCKACECE完成签到 ,获得积分10
1分钟前
Vicky完成签到 ,获得积分10
1分钟前
1分钟前
丰富的绮山完成签到,获得积分10
1分钟前
烟花应助科研通管家采纳,获得30
1分钟前
Akim应助科研通管家采纳,获得30
1分钟前
123发布了新的文献求助10
1分钟前
段采萱完成签到 ,获得积分10
1分钟前
Ava应助123采纳,获得30
1分钟前
leng完成签到 ,获得积分10
1分钟前
dragonhmw完成签到 ,获得积分10
1分钟前
烟花应助依居采纳,获得10
1分钟前
玉一一完成签到 ,获得积分10
1分钟前
哈拉斯完成签到,获得积分10
1分钟前
1分钟前
依居发布了新的文献求助10
1分钟前
蓝胖胖蓝完成签到,获得积分10
1分钟前
2分钟前
稀松完成签到,获得积分10
2分钟前
2分钟前
陌子完成签到 ,获得积分10
2分钟前
zjq完成签到 ,获得积分10
2分钟前
2分钟前
飞云完成签到 ,获得积分10
2分钟前
铁妹儿完成签到 ,获得积分10
3分钟前
SHD完成签到 ,获得积分10
3分钟前
煜琪完成签到 ,获得积分10
3分钟前
高贵逍遥完成签到 ,获得积分10
3分钟前
wyh295352318完成签到 ,获得积分10
3分钟前
jie完成签到 ,获得积分10
3分钟前
さくま完成签到,获得积分10
3分钟前
啥时候能早睡完成签到 ,获得积分10
3分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052644
求助须知:如何正确求助?哪些是违规求助? 2709863
关于积分的说明 7418252
捐赠科研通 2354395
什么是DOI,文献DOI怎么找? 1246007
科研通“疑难数据库(出版商)”最低求助积分说明 605951
版权声明 595921