电离辐射
体外
体内
辐照
内皮干细胞
细胞生物学
生物
内皮
癌症研究
化学
分子生物学
生物化学
内分泌学
遗传学
物理
核物理学
作者
Karol Jelonek,Anna Walaszczyk,Dorota Gabryś,Monika Pietrowska,Chryso Kanthou,Piotr Widłak
出处
期刊:Acta Biochimica Polonica
[Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)]
日期:2011-08-31
卷期号:58 (3)
被引量:23
标识
DOI:10.18388/abp.2011_2253
摘要
Cardiovascular disease is recognized as an important clinical problem in radiotherapy and radiation protection. However, only few radiobiological models relevant for assessment of cardiotoxic effects of ionizing radiation are available. Here we describe the isolation of mouse primary cardiac endothelial cells, a possible target for cardiotoxic effects of radiation. Cells isolated from hearts of juvenile mice were cultured and irradiated in vitro. In addition, cells isolated from hearts of locally irradiated adult animals (up to 6 days after irradiation) were tested. A dose-dependent formation of histone γH2A.X foci was observed after in vitro irradiation of cultured cells. However, such cells were resistant to radiation-induced apoptosis. Increased levels of actin stress fibres were observed in the cytoplasm of cardiac endothelial cells irradiated in vitro or isolated from irradiated animals. A high dose of 16 Gy did not increase permeability to Dextran in monolayers formed by endothelial cells. Up-regulated expression of Vcam1, Sele and Hsp70i genes was detected after irradiation in vitro and in cells isolated few days after irradiation in vivo. The increased level of actin stress fibres and enhanced expression of stress-response genes in irradiated endothelial cells are potentially involved in cardiotoxic effects of ionizing radiation.
科研通智能强力驱动
Strongly Powered by AbleSci AI