炎症
荧光素
生物发光成像
髓过氧化物酶
吞噬细胞
生物发光
NADPH氧化酶
体内
吞噬作用
传出细胞增多
免疫学
巨噬细胞
病理
生物
慢性肉芽肿性疾病
医学
细胞生物学
活性氧
荧光素酶
生物化学
体外
酶
生物技术
基因
转染
超氧化物
作者
Jen-Chieh Tseng,Andrew L. Kung
出处
期刊:Chemistry & Biology
[Elsevier]
日期:2012-09-01
卷期号:19 (9): 1199-1209
被引量:72
标识
DOI:10.1016/j.chembiol.2012.08.007
摘要
Inflammation contributes to the pathophysiology of many diseases. In this report, we present noninvasive bioluminescence imaging methods that distinguish acute and chronic inflammation in mouse models. Systemic delivery of luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) enables detection of acute inflammation largely mediated by tissue-infiltrating neutrophils, whose myeloperoxidase (MPO) activity is required for luminol bioluminescence. In contrast, bioluminescence from injection of lucigenin (bis-N-methylacridinium nitrate) closely correlates with late phase and chronic inflammation. Lucigenin bioluminescence is independent of MPO and, instead, requires phagocyte NADPH oxidase (Phox) activity in macrophages. We are able to visualize tissue inflammation resulting from wound healing, bacterial infection, foreign substance implantation, and antitumor immune responses. Given the central role of inflammation in a variety of disorders, we believe these noninvasive imaging methods can help dissect the differential roles of neutrophils and macrophages in a variety of pathological conditions.
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