再髓鞘化
移植
少突胶质细胞
细胞凋亡
白质
病理
体内
程序性细胞死亡
生物
多发性硬化
医学
免疫学
磁共振成像
神经科学
髓鞘
内科学
中枢神经系统
生物化学
放射科
生物技术
作者
Keiya Iijima,Masayoshi Kurachi,Kazuo Shibasaki,Masae Naruse,Sandra Puentes,Hideki Imai,Yuhei Yoshimoto,Masahiko Murakami,Yasuki Ishizaki
摘要
We previously showed that transplantation of brain microvascular endothelial cells (MVECs) greatly stimulated remyelination in the white matter infarct of the internal capsule (IC) induced by endothelin-1 injection and improved the behavioral outcome. In the present study, we examined the effect of MVEC transplantation on the infarct volume using intermittent magnetic resonance image and on the behavior of oligodendrocyte lineage cells histochemically. Our results in vivo show that MVEC transplantation reduced the infarct volume in IC and apoptotic death of oligodendrocyte precursor cells (OPCs). These results indicate that MVECs have a survival effect on OPCs, and this effect might contribute to the recovery of the white matter infarct. The conditioned-medium from cultured MVECs reduced apoptosis of cultured OPCs, while the conditioned medium from cultured fibroblasts did not show such effect. These results suggest a possibility that transplanted MVECs increased the number of OPCs through the release of humoral factors that prevent their apoptotic death. Identification of such humoral factors may lead to the new therapeutic strategy against ischemic demyelinating diseases.
科研通智能强力驱动
Strongly Powered by AbleSci AI