A label-free visual aptasensor for zearalenone detection based on target-responsive aptamer-cross-linked hydrogel and color change of gold nanoparticles

ZEN-responsive hydrogel was prepared using ZEN aptamer and a cationic conjugated skeleton which was formed by the electrostatic interaction between acrylamide and poly (diallyldimethylammonium chloride) (PDDA). In the presence of ZEN, the binding of aptamer and ZEN caused the hydrogel to dissociate, releasing the gold nanoparticles (AuNPs) encapsulated in the hydrogel into supernatant, whose color changes serving as response instructions were observed by eyes. To realize low dose visual detection, the TMB-H2O2 was used for quantitative readout by AuNPs released from the hydrogel which can efficiently catalyze the reaction to generate a obvious change.There was a good linear relationship between the changed absorbance and concentration of ZEN within 2.5-100 ng/mL, a detection limit of 0.98 ng/mL, recovery rates of 98.8%-101.3% and 99.8%-101.5% for corns and beer, respectively. This developed sensing strategy would provide a promising application for other mycotoxins by replacing the corresponding aptamer sequences.