伤口愈合
氧气
自愈水凝胶
右旋糖酐
化学
活力测定
充氧
生物医学工程
外科
细胞
高分子化学
色谱法
医学
生物化学
麻醉
有机化学
作者
Wen Ren,Victoria Messerschmidt,Michael S. Tsipursky,Joseph Irudayaraj
出处
期刊:ACS applied nano materials
[American Chemical Society]
日期:2023-07-17
卷期号:6 (14): 13116-13126
被引量:3
标识
DOI:10.1021/acsanm.3c01812
摘要
Herein, we propose an oxygen nanobubbles-embedded hydrogel (ONB-G) with carbopol for oxygenation of wounds to accelerate the wound healing process. We integrate carbopol, hydrogel, and dextran-based oxygen nanobubbles (ONBs) to prepare ONB-G where ONBs can hold and release oxygen to accelerate wound healing. Oxygen release tests showed that the proposed ONB-G could encapsulate oxygen in the hydrogels for up to 34 days; meanwhile, fluorescence studies indicated that the ONB-G could maintain high oxygen levels for up to 4 weeks. The effect of carbopol concentration on the oxygen release capacity and rheological features of the ONB-G were also investigated along with the sterility of ONB-G. HDFa cell-based studies were first conducted to evaluate the viability, proliferation, and revival of cells in hypoxia. Scratch assay and mRNA expression studies indicated the potential benefit for wound closure. Histological evaluation of tissues with a pig model with incision and punch wounds showed that treatment with ONB-G exhibited improved healing compared with hydrogel without ONBs or treated without the gel. Our studies show that dextran-shell ONBs embedded in a gel (ONB-G) have the potential to accelerate wound healing, given its oxygen-holding capacity and release properties.
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