电化学发光
检测点注意事项
再现性
微流控
检出限
线性范围
促黄体激素
生物传感器
化学
色谱法
材料科学
分析化学(期刊)
纳米技术
生物医学工程
激素
医学
生物化学
免疫学
作者
Kaifa Xue,Bin Lin,Shiju Huang,Bolin Cai,Wei Cheng Lai,Tingting Zhan,Yi Liang,Chunsun Zhang
标识
DOI:10.1016/j.snb.2023.134259
摘要
Luteinizing hormone (LH) is a gonadotropin, and its concentration is closely related to the female ovulation cycle. However, the traditional lateral flow method for LH detection has poor sensitivity and sustainability. In this work, a dry chemistry, ultrasensitive microfluidic fiber material-based immunosensor (μFIS) has been firstly proposed for electrochemiluminescence (ECL) point-of-care testing (POCT) of LH. The μFIS was composed of four-layer fiber material (paper and cloth)-based pads, and was combined with closed bipolar electrode-ECL (CBPE-ECL) to quantitatively detect LH. The CBPE-ECL μFIS not only retained the high sensitivity and wide linear range associated with CBPE-ECL, but also reserved the simplicity and practicality of the lateral flow assay. In addition, a self-enhanced intramolecular ECL probe was incorporated into the μFIS for signal amplification. Under optimized conditions, the μFIS had a very low detection limit (0.125 μIU mL-1) and positive linear relationship (0.001–100 mIU mL-1), together with excellent selectivity, reproducibility and storage stability. Moreover, the CBPE-ECL μFIS was applied to detect LH in human clinical serum samples, and the results had a good correlation with those of clinical samples. These results revealed that the CBPE-CEL μFIS had the potential as a high-performance POCT for LH or other biomarkers.
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