同源盒蛋白纳米
生物
雷克斯1
胚胎干细胞
纳米同源盒蛋白
细胞生物学
染色质
诱导多能干细胞
细胞分化
转录因子
干细胞
遗传学
基因
作者
Kazuko Okamoto,Hideaki Fujita,Yasushi Okada,Soya Shinkai,Shuichi Onami,Kuniya Abe,Kenta Fujimoto,Kensuke Sasaki,Go Shioi,Tomonobu M. Watanabe
标识
DOI:10.15252/embj.2022112305
摘要
Abstract Nanog and Oct4 are core transcription factors that form part of a gene regulatory network to regulate hundreds of target genes for pluripotency maintenance in mouse embryonic stem cells (ESCs). To understand their function in the pluripotency maintenance, we visualised and quantified the dynamics of single molecules of Nanog and Oct4 in a mouse ESCs during pluripotency loss. Interestingly, Nanog interacted longer with its target loci upon reduced expression or at the onset of differentiation, suggesting a feedback mechanism to maintain the pluripotent state. The expression level and interaction time of Nanog and Oct4 correlate with their fluctuation and interaction frequency, respectively, which in turn depend on the ESC differentiation status. The DNA viscoelasticity near the Oct4 target locus remained flexible during differentiation, supporting its role either in chromatin opening or a preferred binding to uncondensed chromatin regions. Based on these results, we propose a new negative feedback mechanism for pluripotency maintenance via the DNA condensation state‐dependent interplay of Nanog and Oct4.
科研通智能强力驱动
Strongly Powered by AbleSci AI