星形胶质细胞
PLGA公司
胶质纤维酸性蛋白
硫氧化物9
脑缺血
缺血
化学
医学
纳米颗粒
中枢神经系统
基因表达
材料科学
纳米技术
病理
内科学
生物化学
基因
免疫组织化学
作者
Dong Ho Kim,Hyo Jung Shin,Seung Gyu Choi,Fengrui Qu,Min‐Hee Yi,Choong‐Hyun Lee,Sang Ryong Kim,Hyeong-Geug Kim,Jaewon Beom,Yoon Young Yi,Do Kyung Kim,Eun‐Hye Joe,Hee‐Jung Song,Yonghyun Kim
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2024-01-01
卷期号:16 (2): 833-847
被引量:1
摘要
Astrocytes are highly activated following brain injuries, and their activation influences neuronal survival. Additionally, SOX9 expression is known to increase in reactive astrocytes. However, the role of SOX9 in activated astrocytes following ischemic brain damage has not been clearly elucidated yet. Therefore, in the present study, we investigated the role of SOX9 in reactive astrocytes using a poly-lactic-co-glycolic acid (PLGA) nanoparticle plasmid delivery system in a photothrombotic stroke animal model. We designed PLGA nanoparticles to exclusively enhance SOX9 gene expression in glial fibrillary acidic protein (GFAP)-immunoreactive astrocytes. Our observations indicate that PLGA nanoparticles encapsulated with GFAP:SOX9:tdTOM reduce ischemia-induced neurological deficits and infarct volume through the prostaglandin D2 pathway. Thus, the astrocyte-targeting PLGA nanoparticle plasmid delivery system provides a potential opportunity for stroke treatment. Since the only effective treatment currently available is reinstating the blood supply, cell-specific gene therapy using PLGA nanoparticles will open a new therapeutic paradigm for brain injury patients in the future.
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