Establishing Stable Nitritation in MABR through Aeration Control

Nitrogen removal from municipal wastewater through partial nitritation-denitrification (nitritation) is challenging to accomplish in a membrane-aerated biofilm reactor (MABR) due to the reactor configuration, which potentially interferes with nitrite-oxidizing bacteria inhibition. This study investigated the impact of intermittent aeration on the development and sustenance of nitritation in a lab-scale MABR for the treatment of municipal wastewater. The study was accomplished in four phases (Phases I–IV) using a combination of continuous and intermittent aeration modes with aerated and nonaerated cycles of 10 min (5 on/5 off), 20 min (10 on/10 off), and 25 min (10 on/15 off), respectively, and a constant hydraulic retention time of 2.5 h. Biofilm development and stabilization were completed using a continuous aeration condition (Phase I). Nitrite accumulation rate, nitrate production rate, and ammonium nitrogen removal efficiency achieved in Phases II–IV were, 35%, 12%, and 99%; 76%, 3.4%, and 98%; and 94%, 1%, and 98%, respectively. Intermittent aeration significantly improved total inorganic nitrogen removal efficiency by ∼20%. Between the initiation of intermittent aeration and termination of the study, ammonia-oxidizing bacteria activities within the reactor increased by >150% from 4.53 to 12.6 mgN/h·g volatile suspended solids (VSS). In contrast, nitrite-oxidizing bacteria activities declined by >60% from 1.17 to 0.46 mgN/h·gVSS. The consistent lagging of nitrate production rate behind nitrite accumulation rate, increase in ammonia-oxidizing bacteria activities, and decline in nitrite-oxidizing bacteria activities over the operation period indicates the establishment of nitritation. This study demonstrates that using intermittent aeration, nitritation can be developed and sustained in MABR under mainstream conditions.