炎症体
小胶质细胞
神经炎症
分泌物
细胞生物学
星形胶质细胞
生物
目标2
趋化因子
神经科学
免疫系统
炎症
免疫学
中枢神经系统
生物化学
作者
Audrey Gustin,Mélanie Kirchmeyer,Eric Koncina,Paul Felten,Sophie Losciuto,Tony Heurtaux,Aubry Tardivel,Paul Heuschling,Catherine Dostert
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2015-06-19
卷期号:10 (6): e0130624-e0130624
被引量:282
标识
DOI:10.1371/journal.pone.0130624
摘要
Neuroinflammation is the local reaction of the brain to infection, trauma, toxic molecules or protein aggregates. The brain resident macrophages, microglia, are able to trigger an appropriate response involving secretion of cytokines and chemokines, resulting in the activation of astrocytes and recruitment of peripheral immune cells. IL-1β plays an important role in this response; yet its production and mode of action in the brain are not fully understood and its precise implication in neurodegenerative diseases needs further characterization. Our results indicate that the capacity to form a functional NLRP3 inflammasome and secretion of IL-1β is limited to the microglial compartment in the mouse brain. We were not able to observe IL-1β secretion from astrocytes, nor do they express all NLRP3 inflammasome components. Microglia were able to produce IL-1β in response to different classical inflammasome activators, such as ATP, Nigericin or Alum. Similarly, microglia secreted IL-18 and IL-1α, two other inflammasome-linked pro-inflammatory factors. Cell stimulation with α-synuclein, a neurodegenerative disease-related peptide, did not result in the release of active IL-1β by microglia, despite a weak pro-inflammatory effect. Amyloid-β peptides were able to activate the NLRP3 inflammasome in microglia and IL-1β secretion occurred in a P2X7 receptor-independent manner. Thus microglia-dependent inflammasome activation can play an important role in the brain and especially in neuroinflammatory conditions.
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