Approximating the energy landscape of a two-dimensional bistable gene autoregulation model by separating slow and fast dynamics

The energy landscape is widely used to quantify the stability of multistable nonlinear systems, such as bistable gene regulation networks. In physics, the potential can be obtained through integration only for gradient systems. However, multidimensional nonlinear systems are often nongradient, for which the potential is calculated by decomposing the dynamics to gradient and nongradient parts. This potential is then called a quasipotential. Given that one-dimensional (1D) systems can be regarded as gradient systems, we attempt to separate the two-dimensional (2D) system into two 1D systems working on distinct timescales, and the potential can be easily calculated for the two 1D systems separately. This method is used in this study to estimate the energy landscape of a two-variable gene autoregulation model. This elegant and comprehensive method is accessible for 2D nonlinear systems in which the dynamics can be divided into slow and fast parts.