Photocontrolled Single-/Dual-Site Alternative Fluorescence Probes Distinguishing Detection of H2S/SO2 in Vivo

Herein, a novel fluorescent probe by integrating 4-azide-1,8-naphthalic anhydride and spiropyran was obtained. NT-N₃-SP was capable of specifically monitoring H₂S by azide reduced. Upon irradiation by alternate ultraviolet and visible light, both the structure and emission of spiropyran moiety in NT-N₃-SP can be reversibly tuned. Importantly, the alternation will be interrupted in the presence of SO₂. Additionally, NT-N₃-SP was successfully used for detection of H₂S/SO₂ in cells and mice.