纳米探针
化学
适体
MXenes公司
癌症生物标志物
癌细胞
生物结合
癌症
MUC1号
荧光原位杂交
癌症研究
生物物理学
细胞生物学
纳米技术
计算生物学
分子生物学
粘蛋白
生物化学
生物
材料科学
纳米颗粒
基因
有机化学
染色体
遗传学
作者
Song Wang,Shaohua Wei,Shigong Wang,Xiaohua Zhu,Chunyang Lei,Yan Huang,Zhou Nie,Shouzhuo Yao
标识
DOI:10.1021/acs.analchem.8b05343
摘要
Acquiring multilayer information on diverse biomarkers with different spatial distributions at the cellular level is crucial for monitoring the progression of cancers. Herein, a dual-signal-tagged chimeric DNA-functionalized titanium carbide MXenes nanoprobe (dcDNA–Ti3C2) that responds to biomarkers with different cellular locations from plasma membrane to cytoplasm was designed toward this end. In the presence of cancer biomarkers, including transmembrane glycoprotein mucin 1 (MUC1) and cytoplasmic microRNA-21 (miR-21), the recognition between MUC1 and its aptamer in the dcDNA–Ti3C2 probe induces the separation of TAMRA–MUC1 aptamer from Ti3C2 MXenes, thereby resulting in an increase in red fluorescence; and the hybridization of miR-21 with the hairpin probe triggers the increase of green fluorescence. As a result, dual analysis of MUC1 and miR-21 at low-nanomolar concentrations in vitro, as well as in situ simultaneous imaging of the biomarkers within MCF-7 breast cancer cells, was achieved. The feasibility of the nanoprobe was further demonstrated by monitoring the expression changes of both the biomarkers in cancer cells under different inhibitor combinations. Therefore, this strategy allows us to acquire the expression levels and spatial distributions of different biomarkers in living cells, providing a helpful tool for reliable diagnosis of cancers and basic understanding their progression.
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