京尼平
热重分析
壳聚糖
伤口愈合
核化学
纳米复合材料
材料科学
脚手架
热稳定性
傅里叶变换红外光谱
没食子酸
肿胀 的
化学
组织工程
化学工程
生物医学工程
纳米技术
复合材料
有机化学
外科
医学
抗氧化剂
工程类
作者
Pallavi Shyam Kaparekar,Srinivetha Pathmanapan,Suresh Kumar Anandasadagopan
标识
DOI:10.1016/j.ijbiomac.2020.09.212
摘要
The present study explores the curative efficacy of collagen-fibrin scaffold with Gallic acid loaded Chitosan nanoparticles (GA-CSNPs) in wound healing. GA-CSNPs were synthesized by ionotropic gelation and the incorporation of GA was confirmed with Fourier Transform Infra-Red Spectroscopy (FTIR). Change in the crystal structure of GA was confirmed by X-ray Powder Diffraction (X-PRD) and Differential Scanning Colorimetry (DSC). Surface Electron microscopy (SEM) showed that GA-CSNPs have roughly spherical morphology and mean diameter of 251.3 nm with positive zeta potential. The drug encapsulation was found to be 34.2–73.5%. Col-fibrin scaffolds crosslinked with genipin using cryodesiccation technique showed a sheet-like architecture with 66.78% of crosslinking degree. Scaffolds exhibited porosity of 38.49% and decrease in swelling ratio. Biodegradation study demonstrated controlled degradation with collagenase and Thermogravimetric analysis (TGA) showed excellent thermal stability and sustained drug release property. In vitro and in vivo study results indicate that the group treated with nanocomposite scaffold exhibits enhanced re-epithelialization, accelerated fibroblast cell migration, wound healing and significant wound contraction (p < 0.001) compared to control. Nanocomposite scaffolds also accelerates angiogenesis, hexosamine synthesis, collagen deposition and recruiting immune cells at wound area. These results suggest nanocomposite scaffold values for their use as a promising wound dressing material for better tissue regeneration.
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