药物发现
血脑屏障
生物
药物开发
药品
药理学
计算生物学
神经科学
生物信息学
中枢神经系统
作者
Antje Appelt-Menzel,Sabrina Oerter,Sanjana Mathew,Undine Haferkamp,Carla Hartmann,Matthias Jung,Winfried Neuhaus,Ole Pless
摘要
Translating basic biological knowledge into applications remains a key issue for effectively tackling neurodegenerative, neuroinflammatory, or neuroendocrine disorders. Efficient delivery of therapeutics across the neuroprotective blood-brain barrier (BBB) still poses a demanding challenge for drug development targeting central nervous system diseases. Validated in vitro models of the BBB could facilitate effective testing of drug candidates targeting the brain early in the drug discovery process during lead generation. We here review the potential of mono- or (isogenic) co-culture BBB models based on brain capillary endothelial cells (BCECs) derived from human-induced pluripotent stem cells (hiPSCs), and compare them to several available BBB in vitro models from primary human or non-human cells and to rodent in vivo models, as well as to classical and widely used barrier models [Caco-2, parallel artificial membrane permeability assay (PAMPA)]. In particular, we are discussing the features and predictivity of these models and how hiPSC-derived BBB models could impact future discovery and development of novel CNS-targeting therapeutics. © 2020 The Authors.
科研通智能强力驱动
Strongly Powered by AbleSci AI