代谢组
便秘
代谢组学
肠道菌群
萧条(经济学)
微生物群
厚壁菌
医学
内科学
内分泌学
生物信息学
生物
生物化学
基因
免疫学
16S核糖体RNA
宏观经济学
经济
作者
Xiaojie Liu,Ziyu Zhao,Yuhui Fan,Di Zhao,Yaze Wang,Meng Lv,Xue Qin
摘要
Background Depressed patients are often accompanied with constipation symptoms, and vice versa. However, the underlying mechanisms of such a bidirectional correlation have remained elusive. We aim to reveal the possible correlations between depression and constipation from the perspectives of gut microbiome and plasma metabolome. Methods We constructed the depressed model and the constipated model of rats, respectively. First, we measured the locomotor activity status and the gastrointestinal functions of rats. And then, nuclear magnetic resonance plasma metabolomics was applied to reveal the shared and the unique metabolites of depression and constipation. In addition, 16 S ribosomal RNA gene sequencing was used to detect the impacts of constipation and depression on gut microbiota of rats. Finally, a multiscale and multifactorial network, that is, the ‘phenotypes ‐ differential metabolites ‐ microbial biomarkers’ integrated network, was constructed to visualise the mechanisms of connections between depression and constipation. Results We found that spontaneous locomotor activity and gastrointestinal functions of both depressed rats and constipated rats significantly decreased. Further, eight metabolites and 14 metabolites were associated depression and constipation, respectively. Among them, seven metabolites and four metabolic pathways were shared by constipation and depression, mainly perturbing energy metabolism and amino acid metabolism. Additionally, depression and constipation significantly disordered the functions and the compositions of gut microbiota of rats, and decreased the ratio of Firmicutes to Bacteroidetes. Conclusion The current findings provide multiscale and multifactorial perspectives for understanding the correlations between depression and constipation, and demonstrate new mechanisms of comorbidity of depression and constipation.
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