The Research Progress of CRISPR/Cas9 System-Mediated Gene Editing Combined with CAR-T Tumor Immunotherapy

Abstract. CAR-T cell therapy is a highly promising cancer treatment; however, its application and efficacy are limited by various factors. This paper explores the challenges faced by CAR-T cell therapy, such as antigen escape, treatment side effects, the immunosuppressive microenvironment of solid tumors, and low efficiency of cell migration and infiltration. To overcome these bottlenecks, CRISPR/Cas9 technology can be utilized to enhance CAR-T cells through gene editing, improving their anti-tumor effects. The paper details methods and applications of CRISPR/Cas9 technology in improving CAR-T therapy. Finally, the paper summarizes the current bottlenecks in the field and provides prospects for future development.