安普克
PI3K/AKT/mTOR通路
下调和上调
蛋白激酶B
纤维化
肝硬化
肝纤维化
医学
内分泌学
癌症研究
内科学
化学
信号转导
磷酸化
蛋白激酶A
生物化学
基因
作者
Shanshan Zhang,Binjie Liu,Lan Huang,Rong Zhang,An Shen Lin,Zhongqiu Liu
出处
期刊:Research Square - Research Square
日期:2023-05-30
标识
DOI:10.21203/rs.3.rs-2536417/v1
摘要
Abstract Hepatic fibrosis (HF) could be developed into liver cirrhosis or even hepatocellular carcinoma. Stress has an important role in the occurrence and development of various considerable diseases. However, the effect of a certain degree stress on HF is still controversial. In our study, stress was simulated with regular chronic restraint stress (CRS) and HF model was induced with CCl 4 in mice. We found that CRS was able to attenuate CCl 4 -induced liver injury and fibrosis in mice. Surprisingly, behavioral analysis showed that the mice in the HF group exhibited depression-like behavior. Further, the metabolomic analysis revealed that 119 metabolites and 20 metabolic pathways were altered in mice liver, especially the betaine metabolism pathway. Combined with the results of ingenuity Pathway Analysis (IPA) analysis, the key proteins INSR, PI3K, AKT, and p-AMPK were identified and verified, and the results showed that CRS could upregulate the protein levels and mRNA expression of INSR, PI3K, AKT, and p-AMPK in liver tissues of HF mice. It suggested that CRS alleviated CCl 4 -induced liver fibrosis in mice through upregulation of the INSR/PI3K/AKT/AMPK pathway. Proper stress might be a potential therapeutic strategy for the treatment of chronic liver disease, which provided new insights into the treatment of HF.
科研通智能强力驱动
Strongly Powered by AbleSci AI