Synergetic Effect of β-Cyclodextrin and CuO Nanocomposite for Ultrasensitive Determination of Chloramphenicol in Food

Chloramphenicol (CAP), a broad-spectrum antibiotic, has severely impacted human health and the ecological environment, which brings an increasing demand for an efficient monitoring technology for rapid and sensitive antibiotic residue detection. Herein, a highly sensitive and selective electrochemical sensor was developed for detecting CAP, based on the synergistic effect of β -cyclodextrin ( β -CD) and CuO-nanomaterials modifying glassy carbon electrode (GCE). Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) spectrometry were used for morphological characterization. β -CD/CuO/GCE sensor with high-specific surface area and cavity structure was prepared by modifying GCE for further electrochemical testing. Experimental parameters were optimized using square wave stripping voltammetry. The developed sensor obtains an ultra-wide linear range from 1.0 × 10 −7 mol l −1 to 5.0 × 10 −4 mol l −1 and the limit of detection is 0.5 × 10 −7 mol l −1 . The sensor displays high sensitivity, remarkable stability, and reproducibility; particularly more convenient than the Liquid chromatography-mass spectrometry (LC-MS) verification method. Furthermore, the applicability of the developed β -CD/CuO/GCE sensor was demonstrated by detecting CAP in food samples.