毕赤酵母
生物过程
发酵
生物量(生态学)
生物炼制
生物技术
水解物
生化工程
制浆造纸工业
食品科学
生物
生物化学
生物燃料
重组DNA
水解
工程类
基因
农学
古生物学
作者
Korbinian Sinzinger,Ulrike Obst,Samed Güner,Manuel Döring,Magdalena Haslbeck,Doris Schieder,Volker Sieber
标识
DOI:10.1016/j.jobab.2023.12.005
摘要
The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation. We demonstrate these capabilities by producing a phytase secreted by P. pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days.
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