根瘤菌
生物
液泡
细胞生物学
内共生
截形苜蓿
内膜系统
细胞骨架
微管
植物
细胞质
共生
生物化学
质体
遗传学
叶绿体
高尔基体
基因
内质网
细菌
细胞
作者
Xiaxia Zhang,Qi Wang,Jianming Wu,Meifang Qi,Chen Zhang,Yi‐Ge Huang,Guangda Wang,Huan Wang,Juan Tian,Yong Yu,Da-Song Chen,Youguo Li,Dong Wang,Yijing Zhang,Yongbiao Xue,Zhaosheng Kong
出处
期刊:Nature plants
[Springer Nature]
日期:2022-10-31
卷期号:8 (11): 1275-1288
被引量:6
标识
DOI:10.1038/s41477-022-01261-4
摘要
Symbioses between legumes and rhizobia require establishment of the plant-derived symbiosome membrane, which surrounds the rhizobia and accommodates the symbionts by providing an interface for nutrient and signal exchange. The host cytoskeleton and endomembrane trafficking systems play central roles in the formation of a functional symbiotic interface for rhizobia endosymbiosis; however, the underlying mechanisms remain largely unknown. Here we demonstrate that the nodulation-specific kinesin-like calmodulin-binding protein (nKCBP), a plant-specific microtubule-based kinesin motor, controls central vacuole morphogenesis in symbiotic cells in Medicago truncatula. Phylogenetic analysis further indicated that nKCBP duplication occurs solely in legumes of the clade that form symbiosomes. Knockout of nKCBP results in central vacuole deficiency, defective symbiosomes and abolished nitrogen fixation. nKCBP decorates linear particles along microtubules, and crosslinks microtubules with the actin cytoskeleton, to control central vacuole formation by modulating vacuolar vesicle fusion in symbiotic cells. Together, our findings reveal that rhizobia co-opted nKCBP to achieve symbiotic interface formation by regulating cytoskeletal assembly and central vacuole morphogenesis during nodule development.
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