黄曲霉毒素
活性氧
血红蛋白
细胞凋亡
贫血
真菌毒素
红细胞
药理学
生物
流式细胞术
化学
男科
内分泌学
分子生物学
免疫学
内科学
医学
生物化学
食品科学
作者
Sumit Rajaura,Pooja Chauhan,Harish Chandra,Nitin Bhardwaj
出处
期刊:Toxicon
[Elsevier]
日期:2023-01-01
卷期号:221: 106963-106963
被引量:6
标识
DOI:10.1016/j.toxicon.2022.106963
摘要
Aflatoxin is a naturally occurring mycotoxin that has various toxic effects to humans and various other animals. In the current study, we have investigated the toxic effects of Aflatoxin B1 (AFB1) on erythrocytes in the blood circulation of mice. Mice were administered orally with repeated doses of AFB1 (0.3 mg/kg of body weight three times a week for four weeks). AFB1 administration resulted in sustained anemia and a significant reduction in blood erythrocyte number as well as hemoglobin level was seen at different time schedules. Body weight, erythrocyte count, and Hb were significantly decreased on days 30 and 45 post-AFB1 administration. The reticulocytes proportion in circulation was analyzed by staining the cells with anti-mouse CD71 monoclonal antibody and flow cytometric analysis. The ROS level and apoptotic cell proportion were determined by staining with CM-H2DCFDA and Annexin V antibody. AFB1 treatment leads to an increment in reticulocytes production. A significant increase in reactive oxygen species (ROS) and apoptotic cells were also observed in erythrocytes.
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