核梭杆菌
梭杆菌
活检
炎症性肠病
胃肠病学
医学
溃疡性结肠炎
肠粘膜
微生物学
促炎细胞因子
肠易激综合征
结肠炎
内科学
病理
疾病
生物
炎症
细菌
拟杆菌
牙龈卟啉单胞菌
牙周炎
遗传学
作者
Jaclyn Strauss,Gilaad G. Kaplan,Paul L. Beck,Kevin P. Rioux,Remo Panaccione,Rebekah DeVinney,Tarah Lynch,Emma Allen‐Vercoe
出处
期刊:Inflammatory Bowel Diseases
[Oxford University Press]
日期:2011-09-01
卷期号:17 (9): 1971-1978
被引量:430
摘要
Fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. Given that some strains of F. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (IBD) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with IBD patients; and 2) whether gut-derived F. nucleatum strains from IBD patients showed an increased capacity for invasion.Biopsy material was obtained from 56 adult patients undergoing colonoscopy for colon cancer screening purposes or assessment of irritable bowel syndrome status (34 patients), or to assess for presence of gastrointestinal disease (i.e., IBD or indeterminate colitis, 22 patients). We enumerated Fusobacterium spp. strains isolated from human gut biopsy material in a blinded fashion, and then compared the virulence potential of a subset of F. nucleatum strains using an invasion assay in a Caco-2 model system.Fusobacterium spp. were isolated from 63.6% of patients with gastrointestinal disease compared to 26.5% of healthy controls (P = 0.01). In total, 69% of all Fusobacterium spp. recovered from patients were identified as F. nucleatum. F. nucleatum strains originating from inflamed biopsy tissue from IBD patients were significantly more invasive in a Caco-2 cell invasion assay than strains that were isolated from healthy tissue from either IBD patients or control patients (P < 0.05 to 0.001).This study indicates that colonization of the intestinal mucosa by highly invasive strains of F. nucleatum may be a useful biomarker for gastrointestinal disease.
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