塞来昔布
阿那曲唑
子宫内膜异位症
芳香化酶
医学
细胞凋亡
免疫组织化学
体内
细胞生长
芳香化酶抑制剂
环氧合酶
癌症研究
内科学
病理
内分泌学
药理学
肿瘤科
癌症
生物
乳腺癌
酶
遗传学
生物技术
生物化学
作者
Carla Noemí Olivares,Mariela Andrea Bilotas,Analía Gabriela Ricci,Rosa Ines Barañao,Gabriela Fabiana Meresman
出处
期刊:Reproduction
[Bioscientifica]
日期:2013-02-01
卷期号:145 (2): 119-126
被引量:12
摘要
Endometriosis is a benign gynecological disease. Cyclooxygenase-2 (COX-2) and aromatase proteins have been shown to be overexpressed in eutopic endometrium from women suffering from this disease compared to disease-free women. Furthermore, inhibition of these molecules individually was demonstrated to have antiproliferative and proapoptotic effects both in vitro and in vivo in several models. In this study, the effect of combining celecoxib, a selective COX-2 inhibitor, and anastrozole, an aromatase inhibitor, on the implantation and growth of endometriotic like lesions in a murine model of endometriosis was evaluated. Endometriosis was surgically induced in female BALB/c mice. After 28 days of treatment with celecoxib, anastrozole, or their combination, animals were killed and lesions were counted, measured, excised, and fixed. Immunohistochemistry for proliferating cell nuclear antigen and CD34 was performed for assessment of cell proliferation and vascularization. TUNEL technique was performed for apoptosis evaluation. Celecoxib was the only treatment to significantly reduce the number of lesions established per mouse, their size and vascularized area. In addition, cell proliferation was significantly diminished and apoptosis was significantly enhanced by both individual treatments. When the therapies were combined, they reversed their effects. These results confirm that celecoxib and anastrozole separately decrease endometriotic growth, but when combined they might have antagonizing effects.
科研通智能强力驱动
Strongly Powered by AbleSci AI