生物
氮同化
拟南芥
突变体
转录因子
细胞生物学
转录组
重编程
激酶
硝酸盐
营养感应
信号转导
基因
信号
生物化学
基因表达
生态学
作者
Kun-Hsiang Liu,Yajie Niu,Mineko Konishi,Yue Wu,Hao Du,Hoo Sun Chung,Lei Li,Marie Boudsocq,Matthew McCormack,Shugo Maekawa,Tetsuya Ishida,Chao Zhang,Kevan M. Shokat,Shuichi Yanagisawa,Jen Sheen
出处
期刊:Nature
[Springer Nature]
日期:2017-05-01
卷期号:545 (7654): 311-316
被引量:417
摘要
Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca2+ signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca2+-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca2+ signalling network integrates transcriptome and cellular metabolism with shoot-root coordination and developmental plasticity in shaping organ biomass and architecture.
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