Growth rate method was used to test resistance to Fuji-one, isoprothiolane in 175 effective isolates of Magnapothe grisea, which collected from 26 counties in Guizhou Province. The results showed that resistant isolates existed in major districts of Guizhou Province, but most of them revealed low resistance to Fuji-one. Resistance frequency in Qiandongnan, Guiyang and Zunyi was 10.86%, 10.29 and 8.00%, respectively, which was significantly higher than other districts.