Kinetics of Enzyme Catalysis

Kinetics of Enzyme Catalysis provides an introduction to the fundamentals of understanding an enzyme's catalytic mechanism and how activity is regulated, which is key to understanding biology and many diseases. Kinetics is at the core of enzymology, as it must be for the study of catalysts. Kinetics of Enzyme Catalysis examines simple kinetics and then applies those ideas to enzyme mechanisms, leading to rate equations for several key mechanisms and, as important, illustrating some key principles. A reader should therefore come away empowered with some mathematical tools allowing the analysis of catalytic cycles not discussed here and also with the understanding to predict some behaviors of enzyme kinetics without any math. Methods are discussed in some detail, and with them some considerations for avoiding pitfalls and collecting reliable data. In addition, introductions are presented to the important areas of studying inhibitors, of the origins of the catalytic power of enzymes, and the use of rapid-reaction technology.